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标题：Dynamic and stable pools of apoE differ functionally at the HepG2 cell surface
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作者：Jim W. Burgess ; Yves L. Marcel
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2001
卷号：42
期号：9
页码：1413-1420
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Synthesis of apolipoprotein (apo)E in hepatocytes leads to both secretion and retention in cell surface pools. Inclusion of Brefeldin A to HepG2 cells prompted a rapid decrease of cell surface apoE to about 37% of control values after a 3-h incubation. The t _1/2 for this dynamic pool was estimated to be 15 min. In contrast, a stable fraction of apoE ( t _1/2 > 20 h) was found in association with the extracellular matrix (ECM). Increased content of apoE on the ECM correlated with decreased binding of VLDL. Decreased apoE on the cell surface correlated with increased binding of VLDL to cells. Collectively, this suggests that glycosaminoglycan-bound apoE can occlude binding sites for apoE-containing lipoproteins on glycosaminoglycans. In solid-phase assays, heparin, suramin, and chondroitin sulfates A and B efficiently inhibited the binding of apoE to heparan sulfate proteoglycans, but were unable to displace apoE from this glycosaminoglycan. Finally, decreasing cell surface apoE with suramin subsequently decreased the apoE content on secreted apoB-containing lipoproteins without affecting the overall secretion of apoE or apoB to the extracellular medium. In summary, cell surface apoE comprises both dynamic fractions, which can be donated to newly secreted lipoproteins, and stable fractions, which may act to minimize the unproductive binding of lipoproteins to the ECM. —Burgess, J. W., and Y. L. Marcel. Dynamic and stable pools of apoE differ functionally at the HepG2 cell surface. J. Lipid Res. 2001. 42: 1413–1420.
关键词：apolipoprotein E ; extracellular matrix ; secretion ; glycosaminoglycans