文章基本信息

标题：A new method for HDL particle sizing by polyacrylamide gradient gel electrophoresis using whole plasma
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作者：Mélanie Pérusse ; Agnès Pascot ; Jean-Pierre Després 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2001
卷号：42
期号：8
页码：1331-1334
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Low plasma levels of HDL cholesterol have been associated with an increased risk of coronary heart disease. HDL particles are heterogeneous with respect to size and apolipoprotein content. The objective of the present study was to develop a method to generate lipid-stainable calibrators that would allow the assessment of HDL particle size from whole plasma, using polyacrylamide gradient gel electrophoresis (PAGGE). Lipid-stainable HDL calibrators were obtained by subjecting isolated red blood cells to hemolysis either by freezing at −20 or −80°C overnight or by rapid exposure to liquid nitrogen and mixing of the hemolysis products with plasma aliquots. All three methods were highly reproducible in producing Sudan black lipid-stainable HDL calibrators ranging from 75 to 200 Å. The assessment of HDL particle size with these lipid-stainable HDL calibrators was also highly reproducible, with a coefficient of variation below 5.5%. These lipid-stainable HDL calibrators simplify the assessment of HDL particle size by PAGGE using whole plasma, without the need for costly, time-consuming ultracentrifugation procedures. —Pérusse, M., A. Pascot, J-P. Després, C. Couillard, and B. Lamarche. A new method for HDL particle sizing by polyacrylamide gradient gel electrophoresis using whole plasma. J. Lipid Res. 2001. 42: 1331–1334.
关键词：HDL particle size ; hemolysis ; lipid staining