首页    期刊浏览 2024年12月06日 星期五
登录注册

文章基本信息

  • 标题:Construction of stable coxsackievirus and adenovirus receptor-expressing 3T3-L1 cells
  • 本地全文:下载
  • 作者:David J. Orlicky ; James DeGregori ; Jerome Schaack
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:2001
  • 卷号:42
  • 期号:6
  • 页码:910-915
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:3T3-L1 cells have been used as a model to study the differentiation and physiology of adipocytes. Exogenous expression of proteins in these cells offers the prospect of understanding the protein's function(s) in adipose tissue. Viral vectors, in particular, adenovirus, have proven to be a powerful means for introduction of genes into many cell types. However, we have previously shown that 3T3-L1 cells are inefficiently transduced by adenovirus (Orlicky, D. J., and J. Schaack. 2001. J. Lipid Res. 42: 460–466). To overcome the inefficient transduction, we have stably introduced the gene-encoding coxsackie and adenovirus receptor (CAR), which was modified by deletion of the region encoding the cytoplasmic tail, into 3T3-L1 cells. 3T3-L1 CARΔ1 cells are transduced approximately 100-fold more efficiently than parental 3T3-L1 cells. 3T3-L1 CARΔ1 cells should prove to be a useful tool for examination of exogenous protein expression in fat cells. —Orlicky, D. J., J. DeGregori, and J. Schaack. Construction of stable coxsackievirus and adenovirus receptor-expressing 3T3-L1 cells. J. Lipid Res. 2001. 42: 910–915.
  • 关键词:3T3-L1 ; adenovirus ; transduction ; adipose ; adipocyte
国家哲学社会科学文献中心版权所有