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标题：Modulation of cytosolic phospholipase A2 by PPAR activators in human preadipocytes
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作者：Yan J. Jiang ; Grant M. Hatch ; David Mymin 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2001
卷号：42
期号：5
页码：716-724
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Cytosolic phospholipase A₂ (cPLA₂) is responsible for the release of arachidonic acid, a precursor for eicosanoid biosynthesis, from cellular phospholipids. The objective of this study is to examine the regulation of cPLA₂ by peroxisome proliferator-activated receptor (PPAR) activators in preadipocyte SW872 (SW) cells. PPAR belong to the superfamily of nuclear hormone receptors that heterodimerize with the retinoid X receptor. In this study, the presence of both PPAR α and PPAR γ was confirmed in SW cells by positive identification of their mRNA in the cellular homogenate. Clofibrate, a PPAR α activator, caused an enhancement of ionophore A-23187-induced arachidonate release in SW cells. This increase resulted from an enhancement of cPLA₂ activity, which was caused by an increase in enzyme protein. Clofibrate at lower concentrations (10–200 μM) produced increases in the mRNA levels of cPLA₂ in a dose-response manner. At higher concentrations (>400 μM), clofibrate treatment resulted in the attenuation of the cPLA₂ mRNA level and protein expression. We postulate that clofibrate, acting through the PPAR α, caused an induction in the transcription of cPLA₂ gene, which led to an increase in the cPLA₂ protein. The observed increase in arachidonate release in SW cells appeared to be a direct result of the enhanced cPLA₂ activity. —Jiang, Y. J., G. M. Hatch, D. Mymin, T. Dembinski, E. A. Kroeger, and P. C. Choy. Modulation of cytosolic phospholipase A₂ by PPAR activators in human preadipocytes. J. Lipid Res. 2001. 42: 716–724.
关键词：cPLA₂ ; PPAR α ; clofibrate ; preadipocytes ; arachidonic acid ; SW cells ; COX-2 ; peroxisome proliferator-activated receptor