文章基本信息

标题：Nutritional lipid emulsions modulate cellular signaling and activation of human neutrophils
本地全文：下载
作者：G. Wanten ; S. van Emst-de Vries ; T. Naber 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2001
卷号：42
期号：3
页码：428-436
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Although numerous studies suggest that nutritional lipids modulate human immune responses, the mechanism behind this observation remains unclear. On the basis of the hypothesis that lipids might affect cellular signaling we evaluated the effects of various lipid emulsions on two major pathways involved in neutrophil activation: second messenger (Ca²⁺) mobilization and protein kinase C (PKC) activation. Activation by opsonized yeast particles (serum-treated zymosan; STZ) increased cytosolic [Ca²⁺] ([Ca²⁺]_i) in neutrophils, with an initial slow rise that turned into a fast phase until a plateau was reached. The PKC activator 4-α-phorbol 12-myristate 13-acetate (PMA) markedly increased the initial STZ-induced [Ca²⁺]_i rise. This PMA effect was mimicked by emulsions containing medium-chain triglycerides (MT), but not by long-chain triglycerides (LT) or structured lipids (SL). However, like PMA, all emulsions decreased the STZ-induced [Ca²⁺]_i plateau and all activated purified PKC, suggesting that only MT emulsions activate PKC in the context of the intact cell. MT, like PMA, evoked a leftward shift of the dose-response curve for the STZ-induced [Ca²⁺]_i rise, indicating PKC-dependent sensitization of neutrophils for stimulation by STZ. This study is the first to show that nutritional lipids distinctively modulate cellular signaling and stimulation of neutrophils through effects on calcium mobilization and PKC activation: i ) MT emulsions sensitize neutrophils for STZ in a PKC-dependent manner, and ii ) MT, LT, and SL emulsions all reduce the stimulatory effect of STZ in a nonspecific manner. —Wanten, G., S. van Emst-de Vries, T. Naber, and P. Willems. Nutritional lipid emulsions modulate cellular signaling and activation of human neutrophils. J. Lipid Res. 2001. 42: 428–436.
关键词：immune response ; lipids ; nutrition ; emulsion ; calcium signaling ; protein kinase C