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标题：Induction of acyl-coenzyme A:cholesterol acyltransferase-1 by 1,25-dihydroxyvitamin D3 or 9-cis-retinoic acid in undifferentiated THP-1 cells
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作者：Kyu Kyu Maung ; Akira Miyazaki ; Hisayuki Nomiyama 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2001
卷号：42
期号：2
页码：181-187
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：We have previously shown that acyl-coenzyme A: cholesterol acyltransferase-1 (ACAT-1) protein content increases significantly during the human monocyte-macrophage differentiation process. To gain further insight, we used undifferentiated human monocytic THP-1 cells as a model system with which to examine whether ACAT-1 mRNA and protein content can be increased by treating cells with 1,25-dihydroxyvitamin D₃ [1,25-(OH)₂D₃] or with 9- cis -retinoic acid (9- cis -RA), two agents known to upregulate the expression of various genes during the monocyte-macrophage differentiation process. Immunoblot analysis with anti-human ACAT-1 antibodies revealed that ACAT-1 protein was increased by 2.6-fold, using 1,25-(OH)₂D₃ at a physiological concentration (100 pM). ACAT-1 protein was also increased when using 9- cis -RA, but only at relatively high concentrations (0.1–1 μM). Northern blot analysis revealed that among the four ACAT-1 mRNA transcripts (2.8, 3.6, 4.2, and 7.0 kb) examined, only the 2.8- and 3.6-kb transcripts were selectively increased. On the basis of enzyme assays in vitro, ACAT activity was increased 3.0-fold by using 100 nM 1,25-(OH)₂D₃, and 1.8-fold by using 1 μM 9- cis -RA. Together, our results suggest that 1,25-(OH)₃ participates in ACAT-1 gene expression during the monocyte-macrophage differentiation process. —Maung, K. K., A. Miyazaki, H. Nomiyama, C. C. Y. Chang, T-Y. Chang, and S. Horiuchi. Induction of acyl-coenzyme A:cholesterol acyltransferase-1 by 1,25-dihydroxyvitamin D₃ or 9- cis -retinoic acid in undifferentiated THP-1 cells. J. Lipid Res. 2001. 42: 181–187.
关键词：ACAT ; ACAT-1 ; monocyte-macrophage differentiation