文章基本信息

标题：Sphingosylphosphorylcholine is an activator of transglutaminase activity in human keratinocytes
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作者：Kazuhiko Higuchi ; Makoto Kawashima ; Yutaka Takagi 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2001
卷号：42
期号：10
页码：1562-1570
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：We characterize functional roles of a newly discovered chemical, sphingosylphosphorylcholine (SPC), in the epidermis by elucidating the biological effect of SPC on human keratinocytes in culture. The intracellular calcium level of human keratinocytes was increased by incubation with SPC, but not with sphingosine (SS) or sphingomyelin (SM). The addition of SPC, sphingosine 1-phosphate (SSP), or SS to human keratinocytes at 10 μM concentrations also significantly suppressed DNA synthesis, and SPC, but not SSP, or SS increased the activities of membrane-bound and soluble transglutaminases (TGases). Reverse transcription polymerase chain reaction (RT-PCR) of TGase transcripts revealed that SPC treatment at 10 μM concentrations increased the expression of TGase 1 mRNA. The increased activity of soluble TGase was accompanied by the concomitant activation of cathepsin D as revealed by the increased ratio of mature active form to inactive intermediate form of the protease. Pretreatment of human keratinocytes with pepstatin, a protease inhibitor, blocked the increase in soluble TGase activity induced by treatment with SPC. Consistently, SPC treatment at 1–10 μM concentrations stimulated the cornified envelope formation. These findings suggest that SPC plays an important role in the altered keratinization process of epidermis in skin diseases with high expression of sphingomyelin deacylase, such as atopic dermatitis. —Higuchi, K., M. Kawashima, Y. Takagi, H. Kondo, Y. Yada, Y. Ichikawa, and G. Imokawa. Sphingosylphosphorylcholine is an activator of transglutaminase activity in human keratinocytes. J. Lipid Res. 2001. 42: 1562–1570.
关键词：sphingomyelin deacylase ; atopic dermatitis ; sphingolipid ; ceramide