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标题：A sandwich enzyme-linked immunosorbent assay for human serum paraoxonase concentration
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作者：Takeshi Kujiraoka ; Tomoichiro Oka ; Mitsuaki Ishihara 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2000
卷号：41
期号：8
页码：1358-1363
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Serum paraoxonase (PON) is associated with plasma high density lipoproteins, and prevents the oxidative modification of low density lipoproteins. We have developed a sensitive sandwich enzyme-linked immunosorbent assay (ELISA), using two monoclonal antibodies against PON, to measure serum PON concentration. The concentration of PON in healthy Japanese subjects was 59.3 ± 1.3 μg/mL (mean ± SEM; n = 87). Serum PON concentrations in relation to the PON 192 genetic polymorphism were: 69.5 ± 2.9 μg/mL in the QQ genotype; 63.0 ± 1.9 μg/mL in the QR genotype; and 52.8 ± 1.7 μg/mL in the RR genotype. Concentrations were significantly lower in the RR than in the QQ genotype ( P P P P —Kujiraoka, T., T. Oka, M. Ishihara, T. Egashira, T. Fujioka, E. Saito, S. Saito, N. E. Miller, and H. Hattori. A sandwich enzyme-linked immunosorbent assay for human serum paraoxonase concentration. J. Lipid Res. 2000. 41: 1358–1363.
关键词：low density lipoproteins ; high density lipoproteins ; phospholipids ; coronary heart disease