文章基本信息

标题：Hepatic lipase deficiency decreases the selective uptake of HDL-cholesteryl esters in vivo
本地全文：下载
作者：Gilles Lambert ; Marcelo J. A. Amar ; Pascal Martin 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2000
卷号：41
期号：5
页码：667-672
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Recent in vitro studies have provided evidence that hepatic lipase (HL) facilitates the selective uptake of HDL cholesteryl esters (CE), but the in vivo physiological relevance of this process has not been demonstrated. To evaluate the role that HL plays in facilitating the selective uptake of HDL-CE in vivo, we studied the metabolism of [³H]CEt, ¹²⁵I-labeled apolipoprotein (apo) A-I, and ¹³¹I-labeled apoA-II-labeled HDL in HL-deficient mice. Kinetic analysis revealed similar catabolism of ¹²⁵I-labeled apoA-I (as well as ¹³¹I-labeled apoA-II) in C57BL controls and HL deficient mice, with fractional catabolic rates (FCR) of 2.17 ± 0.15 and 2.16 ± 0.11 d⁻¹ (2.59 ± 0.14 and 2.67 ± 0.13 d⁻¹, respectively). In contrast, despite similar hepatic scavenger receptor BI expression, HL-deficient mice had delayed clearance of [³H]CEt compared to controls (FCR = 3.66 ± 0.29 and 4.41 ± 0.18 d⁻¹, P 3H]CEt in HL-deficient mice (62.3 ± 2.1% of total) was significantly less than in controls (72.7 ± 3.0%), while the [³H]CEt remaining in the plasma compartment increased (20.7 ± 1.8% and 12.6 ± 0.5%) ( P —Lambert, G., M. J. A. Amar, P. Martin, J. Fruchart-Najib, B. Föger, R. D. Shamburek, H. B. Brewer, Jr., and S. Santamarina-Fojo. Hepatic lipase deficiency decreases the selective uptake of HDL-cholesteryl esters in vivo. J. Lipid Res. 2000. 41: 667–672.
关键词：reverse cholesterol transport ; apoA-I ; apoA-II ; SR-BI