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标题：Measurement of cholesterol gallstone growth in vitro
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作者：A. Andre van den Berg ; Jaap D. van Buul ; J. Donald Ostrow 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2000
卷号：41
期号：2
页码：189-194
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Methods to study growth of gallstones in the laboratory have not been reported. We here present such a method. Human cholesterol gallstones were harvested from patients with multiple nearly identical stones. The gallstones were washed and added to supersaturated model biles and the formation of cholesterol crystals and the increases in mass of human cholesterol gallstones were studied concurrently, over a period of weeks, using nephelometry and a microbalance, respectively. All stones incubated in model biles supersaturated with cholesterol increased in mass. Increases in the degree of supersaturation of cholesterol in the model biles resulted in increased growth of stones. The mass increases, the growth rates, and the spatial orientation of accreted crystalline cholesterol differed among various stone types. The kinetics and structures of stone growth were similar when the stones were incubated in supersaturated, native, human gallbladder biles. The structure of accreted cholesterol was the same as found on the surface of some human gallstones that were harvested during apparent active growth in situ. This simple method allows accurate measurements of stone growth in vitro, in patterns that mimic stone growth in vivo, and is useful for studies on the relationships of gallstone growth and the kinetics of cholesterol crystallization. —van den Berg, A. A., J. D. van Buul, J. D. Ostrow, and A. K. Groen. Measurement of cholesterol gallstone growth in vitro. J. Lipid Res. 2000. 41: 189–194.
关键词：gallstone ; growth ; cholesterol crystals ; bile