文章基本信息

标题：A simple and highly sensitive radioenzymatic assay for lysophosphatidic acid quantification
本地全文：下载
作者：Jean Sébastien Saulnier-Blache ; Alexia Girard ; Marie-Françoise Simon 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2000
卷号：41
期号：12
页码：1947-1951
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The objective of the present work was to develop a simple and sensitive radioenzymatic assay to quantify lysophosphatidic acid (LPA). For that, a recombinant rat LPA acid acyltransferase (LPAAT) produced in Escherichia coli was used. In the presence of [¹⁴C]oleoyl-CoA, LPAAT selectively catalyzes the transformation of LPA and alkyl-LPA into [¹⁴C]phosphatidic acid. Acylation of LPA was complete and linear from 0 to 200 pmol with a minimal detection of 0.2 pmol. This method was used to quantify LPA in butanol-extracted lipids from bovine sera, as well as from human and mouse plasma. This radioenzymatic assay represents a new, simple, and highly sensitive method to quantify LPA in various biological fluids.— Saulnier-Blache, J. S., A. Girard, M-F. Simon, M. Lafontan, and P. Valet. A simple and highly sensitive radioenzymatic assay for lysophosphatidic acid quantification. J. Lipid Res. 2000. 41: 1947–1951.
关键词：radioenzymatic detection ; lysophosphatidic acid acyltransferase ; phosphatidic acid ; serum ; plasma ; [¹⁴C]oleoyl-CoA ; butanol ; recombinant protein ; Escherichia coli ; bioactive phospholipid ; thin-layer chromatography