文章基本信息

标题：Stress-induced alteration in the lipolytic response to β-adrenoceptor agonists in rat white adipocytes
本地全文：下载
作者：Elisângela Farias-Silva ; Dora Maria Grassi-Kassisse ; Valéria Wolf-Nunes 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1999
卷号：40
期号：9
页码：1719-1727
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：We analysed the sensitivity to β-adrenoceptor agonists in epididymal adipose cells from rats submitted to a stress protocol previously reported to induce alterations in sensitivity to catecholamines in cardiac tissue from rats. Food intake and body weight were lower, whereas adipocytes basal lipolysis was higher (control: 0.59 ± 0.04; stress: 1.00 ± 0.11, μmol glycerol/100 mg total lipids/100 min) in stressed compared to control rats. The responses to isoprenaline (pD₂ control: 7.46 ± 0.11; stress: 8.11 ± 0.17), adrenaline (pD₂ control: 5.78 ± 0.20; stress: 6.13 ± 0.18), and salbutamol (pD₂ control: 5.64 ± 0.28; stress: 5.92 ± 0.34) were sensitized, and the lipolytic responses to norepinephrine (pD₂ control: 6.98 ± 0.13; stress: 6.41 ± 0.12) and to BRL37344 (pD₂ control: 8.43 ± 0.19; stress: 7.54 ± 0.21) were desensitized. Responses to the higher concentration (100 μ m ) of isoprenaline (control: 1.80 ± 0.18; stress: 2.24 ± 0.10 μmol glycerol/100 mg total lipids/100 min), epinephrine (control: 1.64 ± 0.17; stress: 2.24 ± 0.14 μmol glycerol/100 mg total lipids/100 min), salbutamol (control: 0.65 ± 0.11; stress: 1.21 ± 0.41 μmol glycerol/100 mg total lipids/100 min), and d-butyryl-cAMP (control: 1.59 ± 0.17; stress: 2.72 ± 0.25) were significantly enhanced in adipocytes from stressed rats. pD₂ or maximum response to CGP12177 were not altered. Supersensitivity to isoprenaline was abolished by 50 n m ICI118,551 but was not modified by 100 n m metoprolol. However, subsensitivity to norepinephrine and to BRL37344 was abolished by 100 n m metoprolol. Our results suggest that in epididymal adipocytes from stressed rats there is a desensitization of the response to adrenoceptor agonists mediated by β₁-adrenoceptors together with a sensitization of the response mediated by β₂-adrenoceptors. β₃-adrenoceptors seem to be resistant to the stress effect. —Farias-Silva, E., D. M. Grassi-Kassisse, V. Wolf-Nunes, and R. C. Spadari-Bratfisch. Stress-induced alteration in the lipolytic response to β-adrenoceptor agonists in rat white adipocytes. J. Lipid Res. 1999. 40: 1719–1727.
关键词：adrenergic receptors ; footshock stress ; epididymal adipocytes ; sensitivity