文章基本信息

标题：Isolated rabbit enterocytes as a model cell system for investigations of chylomicron assembly and secretion
本地全文：下载
作者：Ian J. Cartwright ; Joan A. Higgins
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1999
卷号：40
期号：7
页码：1357-1365
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A method is described for the isolation of viable enterocytes from rabbit small intestine. The procedure can also be used to isolate populations of epithelial cells from the crypt/villus gradient. The isolated enterocytes synthesized and secreted apoB-48 and triacylglycerol in particles of the density of chylomicrons. Secretion was stimulated by addition of bile salt/lipid micelles. Pulse-chase experiments demonstrated that newly synthesized apoB-48 is degraded intracellularly and that degradation is inhibited by provision of lipid micelles, suggesting that regulation of chylomicron assembly and secretion is broadly similar to that of very low density lipoprotein assembly in hepatocytes. This procedure for preparation of isolated enterocytes will provide a useful model system for investigation of the molecular details of chylomicron assembly. —Cartwright, I. J., and J. A. Higgins. Isolated rabbit enterocytes as a model cell system for investigations of chylomicron assembly and secretion. J. Lipid Res. 1999. 40: 1357–1365.
关键词：small intestine ; fat absorption ; crypt/villus gradient ; thymidine kinase ; alkaline phosphatase ; apoB-48 ; triacylglycerol ; micelles ; degradation