文章基本信息

标题：New methods for determining the enantiomeric purity of erythro-sphingosine
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作者：Shengrong Li ; William K. Wilson ; George J. Schroepfer 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1999
卷号：40
期号：4
页码：764-772
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The enantiomeric purity of erythro -sphingosine samples can be determined simply, reliably, and accurately from ¹H or ¹⁹F nuclear magnetic resonance spectra of the α-methoxy-α-(trifluoromethyl)phenylacetate (MTPA) derivative. As little as 0.1% of the minor enantiomer could be observed in a 1-mg sample, and detection limits of 1% and 5% were estimated for samples of 100 μg and 10 μg. The two threo -sphingosine enantiomers and four dihydrosphingosine stereoisomers were also differentiated by this technique, which served as an effective method for assessing the purity of sphingosine and dihydrosphingosine samples. Enantiomeric and diastereomeric purities could also be determined by normal-phase high performance liquid chromatographic analysis of the MTPA derivatives. —Li, S., W. K. Wilson, and G. J. Schroepfer, Jr. New methods for determining the enantiomeric purity of erythro -sphingosine. J. Lipid Res. 1999. 40: 764–772.
关键词：sphingosine ; enantiomeric purity ; MTPA derivative ; NMR ; HPLC