文章基本信息

标题：Characterization of phosphomevalonate kinase: chromosomal localization, regulation, and subcellular targeting
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作者：Lisa M. Olivier ; Ken L. Chambliss ; K. Michael Gibson 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1999
卷号：40
期号：4
页码：672-679
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Phosphomevalonate kinase catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate and was originally believed to be a cytosolic enzyme. In this study we have localized the phosphomevalonate kinase gene to chromosome 1p13–1q22–23 and present a genomic map indicating that the gene spans more than 8.4 kb in the human genome. Furthermore, we show that message levels and enzyme activity of rat liver phosphomevalonate kinase are regulated in response to dietary sterol levels and that this regulation is coordinate with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis. In addition, we demonstrate that phosphomevalonate kinase is a peroxisomal protein which requires the C-terminal peroxisomal targeting signal, Ser-Arg-Leu, for localization to the organelle. —Olivier, L. M., K. L. Chambliss, K. M. Gibson, and S. K. Krisans. Characterization of phosphomevalonate kinase: chromosomal localization, regulation, and subcellular targeting. J. Lipid Res. 1999. 40: 672–679.
关键词：dietary sterols ; 3-hydroxy-3-methylglutaryl CoA ; peroxisomes