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标题：Percent cholesterol absorption in normal women and men quantified with dual stable isotopic tracers and negative ion mass spectrometry
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作者：Matthew S. Bosner ; Louis G. Lange ; William F. Stenson 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1999
卷号：40
期号：2
页码：302-308
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Percent cholesterol absorption was measured in 94 normal subjects aged 17–80 years while consuming diets generally low in cholesterol (mean intake = 226 ± 126 mg/day). A new dual stable isotope method was used where a cholesterol tracer containing 6 extra mass units was given intravenously and another tracer with 5 extra mass units was given orally during a standard test meal. The ratio of tracers in plasma was determined by negative ion mass spectrometry of pentafluorobenzoyl sterol esters. Absorption values ranged widely from 29.0% to 80.1% with mean 56.2 ± 12.1 (SD) %. Cholesterol absorption was significantly increased in African-Americans (63.4 ± 11.8% vs. 55.1 ± 11.9%, P = 0.027) but was similar for women (53.3 ± 11.9%) and men (57.6 ± 12.1%). It was not related to plasma lipoproteins, age, apoE3/E3 or E3/E4 genotype, or chronic dietary intake of energy, fat, or cholesterol quantitated from 7-day food records. However, dietary cholesterol intake was positively related to plasma cholesterol ( P = 0.036) and triglycerides ( P = 0.026). The milligram amount of dietary cholesterol absorbed (but not percent absorption) was positively correlated with fasting plasma insulin ( r = 0.525, P r = 0.367, P = 0.0003) and glucagon ( r = 0.421, P —Bosner, M. S., L. G. Lange, W. F. Stenson, and R. E. Ostlund, Jr. Percent cholesterol absorption in normal women and men quantified with dual stable isotopic tracers and negative ion mass spectrometry. J. Lipid Res. 1999. 40: 302–308.
关键词：cholesterol ; absorption ; spectrum analysis, mass ; diet ; insulin ; glucagon