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标题：Characterization and quantification of serum lipoprotein subfractions by capillary isotachophoresis: relationships with lipid, apolipoprotein, and lipoprotein levels
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作者：Alexandra Schlenck ; Bernard Herbeth ; Gérard Siest 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1999
卷号：40
期号：11
页码：2125-2133
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Human serum lipoproteins are currently defined according to their density as well as according to their electrophoretic mobility. They can be fractionated into discrete subspecies which exhibit variations in their structure and function. Capillary electrophoresis has been suggested to be a potential analytical strategy in understanding metabolic lipoprotein heterogeneity. In a sample of 35 normolipidemic subjects, we analyzed ceramide-labeled serum lipoproteins by capillary isotachophoresis linked to laser-induced fluorescent detection. Capillary isotachophoresis showed advantage to be an automated, rapid (6 min) and reproducible (CV i ) the fast migrating HDL correlated positively with serum apoA-I ( P P ii ) the intermediate migrating HDL involved in HDL-cholesterol delivery and inversely related to LDL particles concentration ( P iii ) the slow migrating preβ₁HDL. Triglyceride level was significantly associated with two fractions: i ) the VLDL fraction correlated positively with apoE serum concentration ( P ii ) the IDL fraction closely and positively associated with apoC-III-containing lipoprotein level ( P P i ) the fast migrating LDL, positively linked to apoB concentration and to LpCIII:B ( P ii ) the slow migrating LDL. Analytical capillary isotachophoresis of fluorescent-stained lipoprotein subfractions might represent an efficient qualitative and quantitative tool which would afford complementary information on lipoprotein metabolism to current clinical lipoprotein analysis.— Schlenck, A., B. Herbeth, G. Siest, and S. Visvikis. Characterization and quantification of serum lipoprotein subfractions by capillary isotachophoresis: relationships with lipid, apolipoprotein, and lipoprotein levels. J. Lipid Res. 1999. 40: 2125–2133.
关键词：lipoprotein subfractions ; capillary isotachophoresis ; HDL-cholesterol ; LDL-cholesterol ; apolipoproteins ; lipoprotein particles