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标题：Differential effects of 17α-ethinylestradiol on the neutral and acidic pathways of bile salt synthesis in the rat
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作者：Nynke R. Koopen ; Sabine M. Post ; Henk Wolters 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1999
卷号：40
期号：1
页码：100-108
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Effects of 17α-ethinylestradiol (EE) on the neutral and acidic biosynthetic pathways of bile salt (BS) synthesis were evaluated in rats with an intact enterohepatic circulation and in rats with long-term bile diversion to induce BS synthesis. For this purpose, bile salt pool composition, synthesis of individual BS in vivo, hepatic activities, and expression levels of cholesterol 7α-hydroxylase (CYP7A), and sterol 27-hydroxylase (CYP27), as well as of other enzymes involved in BS synthesis, were analyzed in rats treated with EE (5 mg/kg, 3 days) or its vehicle. BS pool size was decreased by 27% but total BS synthesis was not affected by EE in intact rats. Synthesis of cholate was reduced by 68% in EE-treated rats, while that of chenodeoxycholate was increased by 60%. The recently identified Δ²²-isomer of β-muricholate contributed for 5.4% and 18.3 % ( P —Koopen, N. R., S. M. Post, H. Wolters, R. Havinga, F. Stellaard, R. Boverhof, F. Kuipers, and H. M. G. Princen. Differential effects of 17α-ethinylestradiol on the neutral and acidic pathways of bile salt synthesis in the rat. J. Lipid Res. 1999. 40: 100–108.
关键词：cholestasis ; estrogens ; bile ; Δ²²-β-muricholate ; cholesterol-7α-hydroxylase ; sterol-27-hydroxylase ; enterohepatic circulation