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标题：Sterols of skin in the normal and triparanol-treated rat
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作者：L. Horlick ; J. Avigan
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1963
卷号：4
期号：2
页码：160-165
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Skin of normal rats and of rats treated with triparanol were incubated with acetate-2-H³ for 2 hr, and the sterols were extracted and separated chromatographically. Treatment with triparanol resulted in grossly visible changes (scaliness, roughness, and atrophy of the skin) and in a marked alteration in the metabolism of skin sterols. There were considerable reductions in the concentrations of compounds with saturated side chains: cholesterol, methostenol, and dihydrolanosterol; and increases in the concentrations of sterols with unsaturated side chains: desmosterol, lanosterol, and sterols tentatively identified as dehydromethostenol and Δ⁷,24-cholestadienol. Isotope data showed that after incubation for 2 hr, a large proportion of the counts were in squalene in skin from both normal and triparanol-treated rats. There was a reduction in the incorporation of radioactivity into the Δ⁵-stenol fraction and an increase in the incorporation into lanosterol and Δ⁷-sterols, in skin from triparanol-treated rats. Triparanol inhibits the enzyme responsible for reduction of the 24,25-double bond in the sterols of rat skin.