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标题：Reduction of phosphatidylcholine hydroperoxide by apolipoprotein A-I: purification of the hydroperoxide-reducing proteins from human blood plasma
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作者：Ryuichi Mashima ; Yorihiro Yamamoto ; Shinichi Yoshimura 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1998
卷号：39
期号：6
页码：1133-1140
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Plasma glutathione peroxidase (GSHPx) has been suggested to reduce submicromolar levels of free fatty acid hydroperoxides and phosphatidylcholine hydroperoxides (PC-OOH), and therefore these hydroperoxides are undetectable in human blood plasma. The capacity for the reduction should be about 2.5 μ m as the level of glutathione in human plasma is about 5 μ m . However, 2 h of aerobic incubation of 58 μ m PC-OOH in human plasma at 37°C resulted in the formation of 36 μ m phosphatidylcholine hydroxide (PC-OH). The presence of PC-OOH-reducing protein other than plasma GSHPx was suggested by the results. a ) The same rates of PC-OOH decay and PC-OH formation were observed in both sera from rats with selenium-deficient and selenium-supplemented diet; b ) the PC-OOH-reducing activity was observed only in the high molecular weight fraction but not in the low molecular weight fraction; and c ) albumin did not work as a reducing substrate of plasma GSHPx. We have isolated two hydroperoxide-reducing protein fractions from human plasma by a sequential purification scheme, comprising an ammonium sulfate precipitation followed by sequential chromatography on anion exchange, hydrophobic interaction, and heparin columns. One of the proteins was identified as apolipoprotein A-I by N-terminal amino acid sequence analysis. Moreover, the hydroperoxide-reducing activity of one of the fractions was inhibited almost completely by the addition of anti-apolipoprotein A-I antibody. These findings demonstrate that apolipoprotein A-I in high density lipoprotein can reduce PC-OOH to PC-OH.—Mashima, R., Y. Yamamoto, and S. Yoshimura. Reduction of phosphatidylcholine hydroperoxide by apolipoprotein A-I: purification of th hydroperoxide-reducing proteins from human blood plasma. J. Lipid Res. 1998. 39: 1133–1140.
关键词：phosphatidylcholine hydroxide ; selenium-deficient ; selenium-supplemented ; plasma glutathione peroxidase ; high density lipoprotein ; peroxidase