文章基本信息

标题：Measurement of plasma glycerol specific activity by high performance liquid chromatography to determine glycerol flux
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作者：Robert L. Judd ; Rita Nelson ; Samuel Klein 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1998
卷号：39
期号：5
页码：1106-1110
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Previous methods for measuring plasma glycerol specific activity (SA) are suboptimal, making the determination of glycerol kinetics in vivo with radiotracers difficult. A new high performance liquid chromatography (HPLC) method is described that permits the accurate and specific measurement of glycerol SA. The method involves isolation of glycerol from plasma and the formation of a tribenzoyl derivative. Glycerol rate of appearance was measured in five human volunteers using both [2-³H]glycerol and [²H₅] glycerol. There was close agreement between the glycerol appearance rates measured using the two approaches (1.66 ± 0.14 vs. 1.70 ± 0.10 μmol·kg^-1· min^-1, respectively, P = NS). This HPLC method offers improved specificity over existing methods of measuring glycerol turnover using radiotracers.— Judd, R. L., R. Nelson, S. Klein, M. D. Jensen, and J. M. Miles. Measurement of plasma glycerol specific activity by high performance liquid chromatography to determine glycerol flux. J. Lipid Res. 1998. 39: 1106–1110.
关键词：lipolysis ; HPLC ; stable isotopes ; radioisotopes