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标题：A 13CO2 breath test to assess the metabolism of triglyceride-rich lipoprotein remnants in mice
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作者：Ian J. Martins ; Trevor G. Redgrave
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1998
卷号：39
期号：3
页码：691-698
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Remnant-like emulsions were prepared with lipid compositions similar to remnants derived from triacylglycerol-rich lipoproteins. When injected into the bloodstream of conscious mice the remnant-like emulsions labeled with cholesteryl[¹³C]oleate were metabolized in the liver and the appearance of ¹³CO₂ in the breath was measured. In control mice injected with remnant-like emulsions labeled with cholesteryl[¹³C]oleate, enrichment of ¹³CO₂ in the breath peaked at 45 min and then decreased markedly by 3 h. In apoE-deficient (–/–) mice no enrichment was found and in low density lipoprotein receptor (LDLr)-deficient (–/–) mice the appearance of ¹³CO₂ in the breath was markedly decreased. These findings were consistent with the ability of the breath test to detect defects in remnant metabolism. The breath test was useful in detecting a defect in remnant metabolism in LDLr heterozygote (+/–) mice, in which the appearance of ¹³CO₂ in the breath was less by 45 min but remained elevated for the duration of the experiment when compared with control mice. In hepatic lipase-deficient (–/–) mice no defect in remnant metabolism was found. Under fasting conditions, the enrichment of ¹³CO₂ in the breath after injection of emulsion was markedly increased when compared with fed mice, indicating that the metabolism of the injected remnant-like emulsion was probably competed for by post-prandial particles under fed conditions. Our findings show that a ¹³C breath test can be used to assess the metabolism of remnants. The test provides a useful and sensitive method for non-invasive testing of remnant metabolism in experimental animals. —Martins, I. J., and T. G. Redgrave. A ¹³CO₂ breath test to assess the metabolism of triglyceride-rich lipoprotein remnants in mice. J. Lipid Res. 1998. 39: 691–698.
关键词：cholesteryl oleate ; emulsion ; triolein ; triglyceride-rich lipoproteins ; remnants ; cholesterol