文章基本信息

标题：Cholesterol transport between cells and high density lipoprotein subfractions from obese and lean subjects
本地全文：下载
作者：Takayuki Sasahara ; Paul Nestel ; Noel Fidge 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1998
卷号：39
期号：3
页码：544-554
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：We studied the pathway of cholesterol efflux from fibroblasts by testing plasma samples from obese and lean subjects. Plasma samples were incubated with [³H]cholesterol-labeled human skin fibroblasts for 1 h to ensure uniform labeling of all of the high density lipoprotein (HDL) subfractions. Supernatants were then transferred to unlabeled cells and the displacement of labeled cholesterol within HDL subfractions by unlabeled cellular cholesterol was analyzed in short-term experiments. Plasma samples of obese subjects were characterized by a lower content of total apolipoprotein A-I (apoA-I) and α₁-HDL and a lower overall capacity to take up labeled cholesterol. In plasma of lean subjects, preβ₂-HDL and α₁-HDL appeared to be the most active particles in the initial uptake of unlabeled cellular cholesterol. By contrast, in plasmas of obese subjects, the preβ₁-HDL appeared to be most active in taking up unlabeled cellular cholesterol and transferring [³H]cholesterol. There were negative correlations between body mass index (BMI) and apoA-I and α₁-HDL concentrations, and with the apparent increments of cellular cholesterol uptake within preβ₂-HDL and α₁-HDL, as well as with the overall capacity to promote cholesterol efflux. By contrast, BMI was positively correlated with the apparent increment in cellular cholesterol within preβ₁-HDL. While cholesterol efflux was correlated with total plasma apoA-1, there were no such correlations with the concentration of any individual HDL subfraction. We conclude that the pattern of cholesterol transfer between fibroblasts and high density lipoprotein particles is influenced by body fatness and may be a factor in the abnormal metabolism of HDL in obesity.— Sasahara, T., P. Nestel, N. Fidge, and D. Sviridov. Cholesterol transport between cells and high density lipoprotein subfractions from obese and lean subjects. J. Lipid Res. 1998. 39: 544–554.
关键词：cholesterol efflux ; obesity ; high density lipoprotein ; preβ-HDL ; apolipoprotein A-I ; human plasma ; lipoproteins ; reverse cholesterol transport