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标题：VLDL-triglyceride production after alcohol ingestion, studied using [2-13C1] glycerol
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作者：Scott Q. Siler ; Richard A. Neese ; Elizabeth J. Parks 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1998
卷号：39
期号：12
页码：2319-2328
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：We used [2-¹³C₁]glycerol to characterize very low density lipoprotein (VLDL)-triglyceride kinetics and intrahepatic glycerol metabolism in normal men (n = 4) after alcohol (EtOH) ingestion. [2-¹³C₁]glycerol was infused before and after the consumption of 48 g EtOH or a placebo. Three additional subjects also received [1-¹³C₁]acetate in addition to the [2-¹³C₁]glycerol with EtOH treatment. Incorporation of tracer into the glycerol or fatty acid moiety of VLDL-triglyceride was measured by gas chromatography–mass spectrometry and used to calculate VLDL-triglyceride production rates. Intrahepatic triose-phosphate enrichments were also calculated based on mass isotopomer distribution analysis of plasma glucose. There was no difference in VLDL-triglyceride production rates after 48 g EtOH (11.9 ± 3.7 mg/kg/h) or placebo (14.7 ± 3.3 mg/kg/h). The VLDL-triglyceride rate constants calculated by kinetic modeling using the glycerol and acetate tracers in the combined isotope infusion subjects were very closely correlated ( r ² = 0.94). The peak VLDL-glycerol enrichments after EtOH were 22.5 ± 3.3% versus 7.6 ± 0.8% after placebo ( P P —Siler, S. Q., R. A. Neese, E. J. Parks, and M. K. Hellerstein. VLDL-triglyceride production after alcohol ingestion studied using [2-¹³C₁]glycerol. J. Lipid Res. 1998. 39: 2319–2328.
关键词：MIDA ; alcohol ; glycerol phosphate ; hypertriglyceridemia ; kinetic modeling