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标题：Dietary modification of high density lipoprotein phospholipid and influence on cellular cholesterol efflux
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作者：Kristin L. Gillotte ; Sissel Lund-Katz ; Margarita de la Llera-Moya 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1998
卷号：39
期号：10
页码：2065-2075
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：African green monkeys fed fat-specific diets served as a model to investigate the effect of phospholipid acyl chain modification on high density lipoprotein (HDL)-mediated cellular cholesterol efflux. Diets enriched in saturated, monounsaturated, n–6 polyunsaturated, or n–3 polyunsaturated fats were provided during both low cholesterol and cholesterol-enriched stages; sera and HDL₃ samples were obtained at specific points during the treatment period. Analysis of the HDL phospholipid composition revealed significant acyl chain modification, consistent with the respective fat-specific diet. Cholesterol efflux from mouse L-cell fibroblasts to HDL₃ isolated from the specific diet groups was measured and revealed no differences in the abilities of the particles to accept cellular cholesterol; determination of the bidirectional flux of cholesterol between the cells and HDL₃ species further demonstrated no effect of phospholipid acyl chain modification on this process. The effects of dietary modification of phospholipid acyl chains on cellular cholesterol efflux were directly examined by isolating the HDL phospholipid and combining it with human apolipoprotein A-I to form well-defined reconstituted HDL particles. These complexes did not display any differences with respect to their ability to stimulate cellular cholesterol efflux. Incubations with 5% sera further confirmed that the fat-specific diets do not influence cholesterol efflux. These results suggest that the established influences of specific dietary fats on the progression of atherosclerosis are due to effects on cholesterol metabolism other than the efflux of cellular cholesterol in the first step of reverse cholesterol transport. —Gillotte, K. L., S. Lund-Katz, M. de la Llera-Moya, J. S. Parks, L. L. Rudel, G. H. Rothblat, and M. C. Phillips. Dietary modification of high density lipoprotein phospholipid and influence on cellular cholesterol efflux. J. Lipid Res. 1998. 39: 2065–2075.
关键词：acyl chain ; monounsaturated ; saturated ; polyunsaturated