文章基本信息

标题：Raman microspectroscopy of intracellular cholesterol crystals in cultured bovine coronary artery endothelial cells.
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作者：S R Hawi ; K Nithipatikom ; E R Wohlfeil 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1997
卷号：38
期号：8
页码：1591-1597
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Raman microspectroscopy is presented as a promising technique for the in situ characterization of intracellular cholesterol crystals. Crystal characterization is the first step in investigating the effects of various stimuli on their formation and in determining their role in the development of atherosclerosis. Treatment of cultured bovine coronary artery endothelial cells with 22-hydroxycholesterol (220HC) stimulated the production of intracellular crystals, a phenomenon that did not occur in the absence of viable cells. These crystals were identified as a combination of the 220HC starting material and cholesterol. The best fit to the average Raman spectrum of the microscopic crystals was achieved with a combination of 70% Raman contribution from 220HC and 30% from cholesterol. GC/MS analysis of the crystals confirmed the presence of both compounds. These results demonstrate the potential of Raman microspectroscopy as a powerful tool in lipid research, particularly for the in situ characterization of intracellular crystals.