文章基本信息

标题：Selection of monoclonal antibodies for linear epitopes of an apolipoprotein yields antibodies with comparable affinity for lipid-free and lipid-associated apolipoprotein.
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作者：L K Curtiss ; C L Banka
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1996
卷号：37
期号：4
页码：884-892
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A direct approach is described for generating human apolipoprotein (apo) A-I-specific monoclonal antibodies with defined linear epitopes. The antibodies have comparable binding affinity for delipidated apoA-I and apoA-I on high density lipoproteins (HDL). The antibodies were obtained by immunizing mice with HDL, screening the fusions first for antibodies that bind native HDL and second, for antibodies that bind defined apoA-I synthetic peptides. ApoA-I antibody epitopes assigned on the basis of synthetic peptide binding were confirmed by solid phase and fluid phase antibody competition assays. These antibodies, which bind epitopes that encompass greater than 60% of apoA-I, should prove useful for identifying discrete functional domains of apoA-I on HDL.