文章基本信息

标题：In vitro esterification of cholesterol by plasma: the effect of evisceration
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作者：Nathan Brot ; W. J. Lossow ; I. L. Chaikoff 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1962
卷号：3
期号：4
页码：413-415
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The capacity of plasma of eviscerated rats to esterify free cholesterol was compared with that of plasma of normal control rats by incubating the plasma of these rats with free cholesterol-4-C¹⁴ dispersed on Celite and by determining the percentage of labeled sterol in the esterified form at the end of 18 hr. Cholesterol esterification was severely restricted in incubation mixtures prepared with plasma of the eviscerated rats. The restriction could not be accounted for by the presence of inhibitors nor by the absence of heat-stable activators. On the basis of these as well as other observations, it is concluded that the reduced capacity of the plasma of eviscerated rats to esterify cholesterol resulted from removal of the source (probably the liver) of the plasma enzyme or of heat-labile cofactors responsible for the esterification, or both.