文章基本信息

标题：Glycolipid stage-specific embryonic antigens (SSEA-1) in kidneys of male and female C57BL/6J and beige adult mice.
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作者：M A Williams ; S K Gross ; J E Evans 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1988
卷号：29
期号：12
页码：1613-1619
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：SSEA-1 glycolipids from the kidneys of normal male and female as well as beige mutant mice were isolated and their structures were examined by component analysis, mass spectrometry, immunoblotting, and permethylation studies. These antigens were shown to be extended globoside derivatives as reported by Sekine et al. (1987. J. Biochem. 101:553-562). Quantitative high performance liquid chromatography analyses revealed that the concentration of SSEA-1 glycolipids were four-to fivefold greater in male than female mice. Essentially no SSEA-1 glycolipids were excreted in the urine of normal male mice and thus are not components of the multilamellar lysosomes normally excreted. Testosterone is known to induce the hypertrophy of proximal tubule cells that involves the formation of multilamellar lysosomes and results in the accumulation and excretion of these bodies and associated lysosomal enzymes and specific glycolipids. The present results indicate that in male mice there is also an increase in subcellular structures that contain SSEA-1 glycolipids. The amount of SSEA-1 glycolipids in male beige mice were greater than in normal mice on a per kidney basis. Thus, the increase is in proportion to the kidney hypertrophy seen in beige mouse kidneys. Beige mutant mice appear to have a primary defect in the excretion of multilamellar lysosomes which produces a secondary hypertrophy with an accompanying increase in SSEA-1 glycolipids.