文章基本信息

标题：Isolation of hepatocellular lipid droplets: the separation of distinct subpopulations.
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作者：J A Ontko ; L W Perrin ; L S Horne 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1986
卷号：27
期号：10
页码：1097-1103
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A new method for the isolation of lipid droplets from rat liver has been devised. The procedure involves tissue homogenization and discontinuous density gradient centrifugation. Six discrete bands of lipid particles, rich in triglyceride and cholesterol, are visible following 30 min of centrifugation at 25,770 g (max) in a swinging bucket rotor. An entire rat liver can be processed in 1-2 hr. Differences in the density of these liver lipid particles correlate with their triglyceride, sterol, phospho-lipid, and protein contents. The separation of these distinct populations of intracellular particulate neutral lipids provides an approach to the study of their origin and metabolism. This procedure may also be useful in the isolation of various populations of lipid-rich particles from other tissues.