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标题：Very small fat cells. II. Initial observations on basal and hormone-stimulated metabolism.
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作者：A Francendese ; F D DeMartinis
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1985
卷号：26
期号：2
页码：149-157
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Very small fat cells (less than 35 micron diameter) and normal large fat cells (greater than 40 micron diameter) were isolated from adult Fischer 344 rat epididymal adipose depots. These very small fat cell preparations were free from normal, large fat cells (40-130 micron diameter) and stromal-vascular elements. Examination by electron microscopy and lipid analysis showed a similarity in overall organization and composition to normal, large fat cells. Incubations with [U-14C]glucose showed that the very small fat cell preparations oxidized glucose in proportion to both cell number and time. These preparations also responded to insulin, increasing [U-14C]glucose oxidation in a manner similar to normal large fat cell preparations (i.e., 2- to 4-fold increases in CO2 production with insulin stimulation). The very small fat cells also incorporated radiolabeled glucose into lipids; but, unlike normal large fat cells, insulin failed to stimulate this process. Glycerol release from very small fat cells was stimulated by lipolytic hormones in a manner similar to these responses in co-isolated large fat cells.