文章基本信息

标题：Pre-packed reverse phase columns for isolation of complex lipids synthesized from radioactive precursors.
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作者：D A Figlewicz ; C E Nolan ; I N Singh 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1985
卷号：26
期号：1
页码：140-144
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Pre-packed reverse phase columns (Bond Elut) were used for the separation of complex lipids, such as phosphatidylcholine, cerebrosides, sulfatides, and gangliosides, from their respective water-soluble radioactive precursors after their in vitro biosynthesis. After an incubation in vitro, the entire reaction mixture is passed through the column, where complex lipids are retained and the hydrophilic radioactive precursors are washed away from the column. The retained lipids are then eluted with a more nonpolar organic solvent. The procedure is shown to be simpler and more efficient than the normally used Folch partitioning method or other procedures.