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标题：Application of high-performance liquid chromatography to assay phylloquinone (vitamin K1) in rat liver.
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作者：Y Haroon ; P V Hauschka
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1983
卷号：24
期号：4
页码：481-484
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A specific and sensitive method based on high-performance liquid chromatography to measure vitamin K1 in rat liver is described. The procedure consists of three chromatographic steps, a preliminary purification of lipid extracts on Sep-Pak silica columns, a preparative stage using adsorption HPLC, and a final reversed-phase HPLC stage in which vitamin K1 is resolved from remaining contaminants and quantified by reference to an internal standard (vitamin K1 2,3-epoxide). The "within-run" coefficient of variation of the assay for vitamin K1 in 12 livers was 11.1% (n = 24). The concentration of vitamin K1 in liver of male rats fed a control diet containing no alfalfa was 8.0 ng/g (wet weight of liver) while female rat liver gave a mean value of 7.3 ng/g (wet weight of liver). Liver from male rats fed chow containing alfalfa gave a mean value of 44 ng K1/g (wet weight of liver).