文章基本信息

标题：Acetonitrile-hydrochloric acid hydrolysis of gangliosides for high performance liquid chromatographic analysis of their long chain bases.
本地全文：下载
作者：H Kadowaki ; E G Bremer ; J E Evans 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1983
卷号：24
期号：10
页码：1389-1397
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：An improved method for the hydrolysis of long chain bases from gangliosides with aqueous acetonitrile-HCl is described. The long chain bases released from brain gangliosides were derivatized with biphenylcarbonylchloride and resolved by high performance liquid chromatography on a C18 reversed-phase column. Components of individual peaks were identified by gas-liquid chromatography-mass spectrometry as their trimethylsilyl derivatives. The acetonitrile-HCL hydrolysis procedure resulted in no formation of O-methyl ethers of long chain bases and a significant decrease in the level of secondary products.