文章基本信息

标题：Loss of human serum apolipoproteins C and E during manipulation of diluted solutions.
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作者：L Holmquist
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1982
卷号：23
期号：2
页码：357-359
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The adsorption of human serum very low density apolipoproteins C-I, C-II, C-III, and E to the interior surfaces or Beckman Airfuge plastic tubes and glass pipettes has been studied. 125I-Labeled apolipoprotein C-III-1 (1 microgram/ml) was used as a model compound to study the adsorption. After 60 min about 45% was bound to the tube walls, and 5-10% bound rapidly to the pipette used for removing the solution from the tube. Addition of increasing amounts of non-labeled apolipoprotein C-I, C-II, C-III, or E to the tubes reduced the amount of labeled C-III on the tube walls, indicating a strong affinity of these apolipoproteins for the surface. The results demonstrate the importance of careful control of the different steps in immunological ultramicroquantitation methods, that might be influenced by adsorption-losses of the apolipoproteins.