文章基本信息

标题：Enzymatic determination of bile acids from liver cells with 3 alpha-hydroxysteroid dehydrogenase--a warning.
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作者：C A Barth ; K Wirthensohn
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1981
卷号：22
期号：6
页码：1025-1027
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：3 alpha-Hydroxysteroid dehydrogenase was used to monitor bile acid production by rat hepatocyte suspensions. Several observations suggested that the method lacked specificity and detected some other metabolites besides bile acids. This was due to a malate dehydrogenase activity present in four different commercial enzyme preparations of 3 alpha-hydroxysteroid dehydrogenase. It is concluded that considerable error can be caused by this contaminating malate dehydrogenase activity, especially in the case of low bile acid concentration in the sample.