文章基本信息

标题：Separation of sulfated from non-sulfated serum bile acids without the use of Sephadex columns.
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作者：G Lepage ; C C Roy ; A M Weber 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1981
卷号：22
期号：4
页码：705-711
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Methods currently in use for the quantitative measurement of sulfated serum bile acids usually lead to low recoveries. The present technique uses two internal standards and involves enzymatic hydrolysis followed by extraction of the non-sulfated fraction in ether at pH 4.6. The sulfated fraction is then solvolysed before gas-liquid chromatographic analysis of the individual bile acids of both fractions. Using 200 microliters of serum, recoveries of more than 81% of sulfated bile acids were achieved. This method is of particular significance in view of recent evidence showing that certain sulfated bile acids are cholestatic.