文章基本信息

标题：A rapid and simple method for measurement of total protein in very low density lipoproteins by the Lowry assay.
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作者：M L Kashyap ; B A Hynd ; K Robinson 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1980
卷号：21
期号：4
页码：491-495
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：When total protein in very low density lipoprotein samples is measured by the method of Lowry et al. (1951. J. Biol. Chem 193:265-275), turbidity remains in the final color reaction. Addition of 0.1 ml of 2.5% (v/v) solution of Triton X-100 removes this turbidity immediately and effectively. This simple modification is faster, less cumbersome, and more economical than removing turbidity with ethyl ether or chloroform. Addition of 0.1 ml of 2.5% (w/v) sodium dodecyl sulfate to the final reaction mixture is also effective in removing turbidity and can be used as an alternative method.