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  • 标题:Biliary bile acids, bile alcohols, and sterols of Alligator mississippiensis.
  • 本地全文:下载
  • 作者:G S Tint ; B Dayal ; A K Batta
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1980
  • 卷号:21
  • 期号:1
  • 页码:110-117
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:Bile from Alligator mississippiensis was found to contain a mixture of more than twenty bile acids, bile alcohols, and neutral sterols. Bile acids and bile alcohols were purified by reversed-phase high performance liquid chromatography and thin-layer chromatography. Concentrations were measured by gas-liquid chromatography on 1% HiEFF-8BP and identifications were made by mass spectrometry. The major neutral sterols consisted of 98% cholesterol and 0.8% cholestanol. Bile acids recovered from the acidic fraction were 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestanoic acid (61%), 3 alpha, 7 alpha-dihydroxy-5 beta-cholestanoic acid (9%), 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 alpha-cholestanoic acid (8%), and 3-oxo-7 alpha, 12 alpha-dihydroxy-5 beta-cholestanoic acid (10%). Other C27 bile acids identified were: 3 alpha, 12 alpha-dihydroxy-5 beta-cholestanoic acid, 7-oxo-3 alpha, 12 alpha-dihydroxy-5 beta-cholestanoic acid, and 3-oxo-7 alpha, 12 alpha-dihydroxy-5 alpha-cholestanoic acid. Small quantities of C24 cholic, 5 alpha-cholic, chenodeoxycholic, and ursodeoxycholic acids were also detected, as were trace amounts of the C27 bile alcohols 5 alpha-cholestane-3 alpha, 7 alpha, 12 alpha, 26-tetrol, and 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha, 25-tetrol. These results suggest that the alligator is acpable of synthesizing both the 5 alpha and 5 beta-isomers of the C27 bile acids. The small amounts of the C24 bile acids present might originate either from C27 bile acid or bile alcohol precursors or from exogenous sources.
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