文章基本信息

标题：Quantification of adipocyte free and esterified cholesterol using liquid gel chromatography.
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作者：B R Krause ; A D Hartman
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1978
卷号：19
期号：6
页码：774-777
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A reliable method for the separation of free and esterified cholesterol in adipocyte extracts is described. The procedure uses Sephadex LH-20, a lipophilic dextran gel, with a solvent system of chloroform-hexane 55:45 (v/v). Interference by excess triglyceride, such as that encountered in adipocyte total lipid extracts, was not observed, and overall recovery of both sterols exceeded 98%.