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  • 标题:An improved two dimensional thin-layer chromatography system for the separation of phosphatidylglycerol and its derivatives.
  • 本地全文:下载
  • 作者:B J Poorthuis ; P J Yazaki ; K Y Hostetler
  • 期刊名称:JLR Papers In Press
  • 印刷版ISSN:0022-2275
  • 电子版ISSN:1539-7262
  • 出版年度:1976
  • 卷号:17
  • 期号:4
  • 页码:433-437
  • 语种:English
  • 出版社:American Society for Biochemistry and Molecular Biology
  • 摘要:A two dimensional thin-layer chromatography system has been devised for the improved separations of phosphatidylglycerol and its derivaties, cardiolipin and bis/monoacylglyceryl)phosphate, from the other phospholipid components of tissue total lipid extracts. The system employs silica gel G plates prepared with 0.4 M boric acid. Linear recovery of added phosphatidylglycerol was found, and phosphatidylglycerol did not cochromatograph with N, N-dimethylphosphatidylethanolamine in this system. The phospholipid class composition of various rat tissues and a Morris 7777 hepatoma has been determined and compared with values from the literature.
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