文章基本信息

标题：Isolation of disaturated phosphatidylcholine with osmium tetroxide.
本地全文：下载
作者：R J Mason ; J Nellenbogen ; J A Clements 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1976
卷号：17
期号：3
页码：281-284
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A simple, rapid, inexpensive method for isolating disaturated phosphatidylcholine from adult rat lung has been devised. Total lipids are reacted with osmium tetroxide dissolved in carbon tetrachloride, and the disaturated phosphatidylcholine is isolated on a column of neutral alumina. More than 99% of the fatty acids in the phosphatidylcholine fraction are saturated and 94% of this material migrates as phosphatidylcholine on subsequent thin-layer chromatography.