文章基本信息

标题：Role of sarcoplasmic reticulum phospholipids in calcium ion binding activity
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作者：Joel Steinberg ; E. J. Masoro ; Byung Pal Yu 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1974
卷号：15
期号：6
页码：537-543
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The relationship between sarcoplasmic reticulum phospholipid and Ca²⁺ binding by sarcoplasmic reticulum membraneswas explored. Ca²⁺ bound in the absence of ATP was defined as “ATP-independent Ca²⁺ binding,” and the additional amount of Ca²⁺ bound in the presence of ATP was defined as “ATP-dependent Ca²⁺ binding.” The latter was found to be very sensitive to the loss of sarcoplasmic reticulum phospholipid; the amount of Ca²⁺ bound was reduced when as little as 3% of the phospholipid was destroyed by phospholipase C. Further destruction of membrane phospholipid up to a 40% loss caused little or no further reduction of this Ca²⁺ binding. However, when the destruction of phospholipid exceeded 40%, further loss of this Ca²⁺ binding occurred, andthere was an almost complete loss of this function when more than 60% of the sarcoplasmic reticulum phospholipid was destroyed.
关键词：phospholipase C ; “ATP-dependent Ca²⁺ binding” ; “ATP-independent Ca²⁺ binding” ; “ATP-dependent, oxalate-promoted Ca²⁺ transport”