文章基本信息

标题：Isolation and characterization of glucosylsphingosine from Gaucher's spleen
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作者：Srinivasa S. Raghavan ; Richard A. Mumford ; Julian N. Kanfer 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1974
卷号：15
期号：5
页码：484-490
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Glucosylsphingosine has been isolated for the first time as a natural constituent from Gaucher's spleen. On thinlayer chromatography, it migrates with authentic glucosylsphingosine, yielding a positive color reaction with ninhydrin for the amino group and with α-naphthol–sulfuric acid for the carbohydrate residue. N -Acylation with palmitic acid gave rise to glucosylceramide, which was cleaved by purified glucosylceramide: β-glucosidase to ceramide. Gas–liquid chromatography of the trimethylsilyl derivative showed a retention time similar to authentic glucosylsphingosine. Gas–liquid chromatographic analysis of the trimethylsilyl derivatives after methanolysis revealed the presence of only glucose and C₁₈-sphingosine. Mass spectral data further supported the structural identity with glucosylsphingosine.
关键词：Gaucher's disease ; thin-layer chromatography ; gas–liquid chromatography ; β-glucosidic linkage