文章基本信息

标题：Separation of the main lipoprotein density classes from human plasma by rate-zonal ultracentrifugation
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作者：J. R. Patsch ; S. Sailer ; G. Kostner 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1974
卷号：15
期号：4
页码：356-366
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The major lipoprotein density classes (chylomicrons-VLDL, LDL, HDL₂ and HDL₃) were isolated from human plasma in a two-step ultracentrifugal procedure using the Ti-14 zonal rotor. The isolation of the two major high density lipoprotein subclasses (HDL₂ and HDL₃) was achieved in a 24-hr run using a nonlinear NaBr gradient in the density range of 1.00-1.40. The lipoproteins with a density 2) without increasing the running time. Furthermore, this procedure proved to be suitable for isolation of plasma lipoproteins from subjects with various types and varying degrees of hyperlipoproteinemia.
关键词：nonlinear density gradient ; continuous linear density gradient ; lipoprotein protein ; lipoprotein lipids