文章基本信息

标题：New colorimetric method for the quantitative estimation of phospholipids without acid digestion
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作者：R. K. Raheja ; Charanjit Kaur ; Ajit Singh 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1973
卷号：14
期号：6
页码：695-697
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A unique colorimetric method for the quantitative determination of phospholipids that does not involve the acid digestion of the lipid is described. The phospholipids, after separation by thin-layer chromatography and elution from the silica gel, are heated with a chromogenic solution that is a modification of a spray reagent formulated by Vaskovsky and Kostetsky (1968, J. Lipid Res. , 9: 396). The absorbance of the colored complex was read at 710 nm, and it followed Beer's law in the range of 1–10 μg of phospholipid phosphorus.
关键词：chromogenic solution ; thin-layer chromatography ; egg yolk phospholipids