文章基本信息

标题：Glycerolipid synthesis in rat adipose tissue. II. Properties and distribution of phosphatidate phosphatase
本地全文：下载
作者：S. C. Jamdar ; H. J. Fallon
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1973
卷号：14
期号：5
页码：517-524
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The properties and subcellular distribution of phosphatidate phosphatase (EC 3.1.3.4) from adipose tissue have been investigated. The enzyme was assayed using both aqueous phosphatidate and membrane-bound phosphatidate as substrates. When measured with aqueous substrate, activity was detected in the mitochondria, the microsomes, and the soluble fraction. Mg²⁺ at low concentration stimulated the phosphatidate phosphatase from soluble and microsomal fractions but had no effect on the mitochondrial phosphatidate phosphatase. At higher concentration Mg²⁺ was inhibitory. In the presence of Mg²⁺, the phosphatidate phosphatase from soluble and microsomal fractions was active against membrane-bound phosphatidate. No activity was demonstrated with membrane-bound substrate in the absence of Mg²⁺. Mitochondria did not contain activity toward the membrane-bound substrate. The rate of utilization of aqueous phosphatidate was always higher than that of membrane-bound substrate. These results indicate that there are at least two different phosphatidate phosphatases in adipose tissue.
关键词：aqueous phosphatidate ; membrane-bound phosphatidate ; Mg²⁺