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标题：Structure determination of the phosphatidylglycerosulfate (diether analog) from Halobacterium cutirubrum
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作者：A. J. Hancock ; M. Kates
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1973
卷号：14
期号：4
页码：422-429
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：A novel phosphosulfolipid has been isolated as the potassium salt from the extremely halophilic bacterium Halobacterium cutirubrum ; its molecular formula was established as C₄₆H₉₃PSK₂. It accounts for 3–4% by weight of the polar lipids and about 6% of the total lipid phosphorus. The phosphosulfolipid yielded inorganic sulfate and 1- sn -phosphatidyl-3′- sn -glycerol (diphytanyl ether analog) in equimolecular proportions after solvolysis in 0.005 n HCl in tetrahydrofuran, and it was thus a sulfate ester of phosphatidylglycerol. The position of the sulfate group was determined by comparison of the infrared and NMR spectra of the salt and the methyl ester of natural phosphosulfolipid with those of synthetic 1- and 2-sulfate esters of phosphatidylglycerol. The results obtained established the structure of the bacterial phosphosulfolipid as 2,3-di- O -(3′ R ,7′ R ,11′ R ,15′-tetramethylhexadecyl)- sn -glycero-1-phosphoryl-3″- sn -glycero-1″-sulfate.
关键词：phosphosulfolipid ; halophilic bacteria ; 1- sn -phosphatidyl-3′- sn -glycero-1′-sulfate ; 1- sn -phosphatidyl-1′- sn -glycero-2′-sulfate ; 1′- sn -phosphatidyl-3′- sn -glycerol ; 1- sn -phosphatidyl-2′-glycerol ; 2,3- O -phytanyl- sn -glycerol ; 1,3-di- O -phytanyl- sn -glycerol ; NMR spectra ; infrared spectra ; thin-layer chromatography ; cation analysis