文章基本信息

标题：Bile acids. XXXVIII. Conversion of 5α-cholestane-3β,7α-diol to allo bile acids by the rat
本地全文：下载
作者：Burton W. Noll ; E. A. Doisy ; William H. Elliott 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：1973
卷号：14
期号：4
页码：385-390
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：5α-[4-¹⁴C, 3α-³H]Cholestane-3β,7α-diol was prepared from individual samples of 5α-[3α-³H]cholestane-3β,7α-diol and 5α-[4-¹⁴C]cholestane-3β,7α-diol, each derived from 3β-acetoxycholest-5-en-7-one. Bile was collected for 11 days from adult male rats, with cannulated bile ducts, that had received intraperitoneally 0.90–0.92 mg of the doubly labeled diol. Bile from the first 10 hr, containing 63% of the administered ¹⁴C and 6% of the ³H, was hydrolyzed, and the bile acids were separated by acetic acid partition chromatography. Allochenodeoxycholic and allocholic acids contained at least 20.6% and 48.6%, respectively, of the ¹⁴C retained in the biliary acids. Small amounts of ¹⁴C (2.5% and 1.9%, respectively) were present in the 3β isomers of these acids, but the tritium content totaled more than half of that found in the bile acid fraction. No evidence was obtained for presence of the extensive quantities of the allomuricholates.
关键词：3β-hydroxy allo bile acids ; allocholic acid ; allochenodeoxycholic acid ; allomuricholates ; 5α-[4-¹⁴C]cholestane-3β,7α-diol ; 5α-[3α-³H]cholestane-3β,7α-diol