出版社:American Society for Biochemistry and Molecular Biology
摘要:An extremely rapid and sensitive assay for lipoprotein lipase activity, suitable for routine determinations, is described. The substrate for the assay is emulsified [2-3H]glyceryl trioleate, activated by serum. The method is based on trichloroacetic acid precipitation of unreacted substrate and measurement of 3H-labeled glycerol.